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Updated: Jun 10, 2025

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
The expression and function of Gpnmb in lymphatic endothelial cells
Trinity A Kronk1, Ernesto Solorzano2, Gabrielle T Robinson3
1Department of Anatomy and Neurobiology, College of Medicine, Northeast Ohio Medical University, Rootstown, OH, USA; Basic and Translational Biomedicine, College of Graduate Studies, Northeast Ohio Medical University, Rootstown, OH, USA; Musculoskeletal Research Group, Northeast Ohio Medical University, Rootstown, OH, USA; Department of Orthopaedics, Akron Children's Hospital, Akron, OH, USA; University Hospitals, Cleveland, OH, USA.
Abstract:
The lymphatic system functions in fluid homeostasis, lipid absorption and the modulation of the immune response. The role of Gpnmb (osteoactivin), an established osteoinductive molecule with newly identified anti-inflammatory properties, has not been studied in lymphangiogenesis. Here, we demonstrate that Gpnmb increases lymphatic endothelial cell (LEC) migration and lymphangiogenesis marker gene expression in vitro by enhancing pro-autophagic gene expression, while no changes were observed in cell proliferation or viability. In addition, cellular spreading and cytoskeletal reorganization was not altered following Gpnmb treatment. We show that systemic Gpnmb overexpression in vivo leads to increases in lymphatic tubule number per area. Overall, data presented in this study suggest Gpnmb is a positive modulator of lymphangiogenesis.
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