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Microbial Corrosion

Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...

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Related Experiment Video

Updated: May 5, 2026

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

Published on: August 18, 2017

Studying microbially induced corrosion on glass using ToF-SIMS.

Gabriel D Parker^1,2, Andrew Plymale³, Jacqueline Hager³

¹Department of Chemistry, University of Illinois Chicago, 845 W Taylor St., Chicago, Illinois 60607.

|October 11, 2024

Summary

This summary is machine-generated.

This study presents a protocol for preparing bacterial biofilms for Time-of-flight secondary ion mass spectrometry (ToF-SIMS) analysis. Centrifugal spinning (CS) desalination effectively reduces media interference, enabling detailed study of microbially induced corrosion (MIC).

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Area of Science:

Environmental Science
Microbiology
Materials Science

Background:

Microbially induced corrosion (MIC) poses environmental risks, particularly concerning radioactive waste glass and material disposal.
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a sensitive technique for analyzing biofilm-substrate interactions.
Effective biofilm preparation is crucial for accurate ToF-SIMS analysis of MIC.

Purpose of the Study:

To develop and compare methods for preparing bacterial biofilms on susceptible substrates for ToF-SIMS analysis.
To evaluate the impact of desalination techniques on sample preparation for ToF-SIMS.
To demonstrate the application of ToF-SIMS in studying MIC on glass surfaces.

Main Methods:

Comparison of three biofilm preparation techniques: no desalination, centrifugal spinning (CS), and water submersion (WS).
Quantitative analysis of media peak reduction and biological signal enhancement using different desalination methods.
Application of ToF-SIMS to analyze chemical compositional changes on glass exposed to *Paenibacillus polymyxa* SCE2 biofilms over 3 months.

Main Results:

Centrifugal spinning (CS) reduced media peaks by up to 99%, while water submersion (WS) reduced them by 55%.
Water submersion (WS) enhanced biological signals, such as fatty acids, by over four times.
ToF-SIMS analysis revealed chemical compositional changes in glass exposed to biofilms, identifying potential MIC products like phosphate phases.

Conclusions:

Centrifugal spinning (CS) is an effective desalination method for ToF-SIMS analysis of biofilms, minimizing media interference without significantly altering biofilm structure.
The developed protocol enables detailed investigation of microbially induced corrosion (MIC) on solid surfaces using ToF-SIMS.
ToF-SIMS can detect MIC-specific chemical alterations on materials exposed to bacterial biofilms over time.