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Updated: Jun 12, 2025

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
Published on: March 12, 2017
Protocol to detect spontaneous termination of yeast RNAPII transcription in vitro
Zhong Han1, Jesper Q Svejstrup1
1Department of Cellular and Molecular Medicine, Panum Institute, Blegdamsvej 3B, University of Copenhagen, 2200 Copenhagen N, Denmark.
Abstract:
We present a protocol for the in vitro detection of spontaneous termination of yeast RNA polymerase II (RNAPII) transcription using bead-immobilized elongation complexes (ECs). We describe the steps for EC assembly, ligation to a long transcription template, and the in vitro elongation and termination reactions. Our protocol has proven successful for identifying spontaneous termination in the yeast CYC1 terminator. For complete details on the use and execution of this protocol, please refer to Han et al.1.
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