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Updated: Jul 15, 2026

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
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Measuring GPCR-Induced Intracellular Calcium Signaling Using a Quantitative High-Throughput Assay

Shree S Kumar1, Kathleen G Mountjoy2

  • 1Faculty of Medical and Health Sciences, Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New Zealand.

Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2024
PubMed
Summary

Researchers developed a new high-throughput method to measure intracellular calcium in live cells. This quantitative assay uses Fura-2/AM in HEK293 cells, enabling robust G-protein-coupled receptor signaling studies.

Keywords:
Calcium mobilizationFluorescenceFura-2 dyeG-protein-coupled receptorsIntracellular calcium quantitationPHERAstar FS plate readerRatiometric

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