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Quantitative Intravital Calcium Imaging in Mouse Kidney.
Andreja Figurek1, Nevena Jankovic1, Andrew M Hall2,3,4
1Institute of Anatomy, University of Zurich, Zürich, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2024
Summary
Disordered intracellular calcium signaling in kidney cells may cause kidney disease. This study details intravital multiphoton imaging in mice to observe real-time calcium activity in renal tubules, aiding disease research.
Area of Science:
- Nephrology
- Cellular Biology
- Physiology
Background:
- Intracellular calcium (Ca2+) regulates solute transport in renal epithelial cells.
- Dysregulated calcium signaling is implicated in the pathogenesis of kidney diseases.
- Understanding renal calcium dynamics is crucial for kidney disease research.
Purpose of the Study:
- To provide a detailed protocol for intravital multiphoton imaging of renal calcium signals.
- To enable real-time observation of calcium activity in specific renal tubule segments.
- To facilitate the study of calcium signaling integration with other cellular processes in vivo.
Main Methods:
- Utilizing transgenic mice with sensitive fluorescent calcium reporters.
- Employing intravital multiphoton microscopy for high-resolution kidney imaging.
- Observing dynamic calcium changes in response to physiological stimuli or disease models.
Main Results:
- Detailed visualization of intracellular calcium dynamics within distinct renal tubule segments.
- Real-time monitoring of cellular activity and its integration with calcium signaling.
- Demonstration of the technique's utility in studying kidney physiology and pathology.
Conclusions:
- Intravital multiphoton imaging is a powerful technique for studying renal calcium signaling.
- This method allows for in vivo investigation of calcium's role in kidney function and disease.
- The provided protocol will advance research into kidney diseases linked to calcium dysregulation.
