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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 10, 2025

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Published on: December 26, 2014

Essential Fluidics for a Flow Cytometer.

Pearlson Prashanth Austin Suthanthiraraj¹, Andrew P Shreve², Steven W Graves²

¹Trauma and Transfusion Medicine Research Center, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania.

Current Protocols

|October 14, 2024

Summary

This summary is machine-generated.

Flow cytometry uses fluid dynamics to analyze particles one by one, enabling high-speed cell analysis and sorting. This technique is crucial for accurate clinical and research measurements across various scientific fields.

Keywords:

FACS cell analysis flow cytometry fluidics fluorescence‐activated cell sorting microfluidics

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Last Updated: Jun 10, 2025

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Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Flow cytometry analyzes particles individually using fluid dynamics.
Optimized for cell-sized particles, it achieves high analysis rates (up to 100 kHz).
Its precision is vital for blood analysis and other applications.

Purpose of the Study:

To provide an overview of fluidic systems in flow cytometry.
To discuss historical and current fluidic approaches.
To explore potential future fluidic systems for analysis and sorting.

Main Methods:

Utilizes fluid dynamics for precise particle positioning in a sensing region.
Employs volumetric sample delivery for accurate particle counting.
Integrates high-throughput sampling technologies for increased sample processing rates.

Main Results:

High analysis rates enable rapid data collection for large cell populations.
Sensitive multiparameter measurements provide detailed cellular information.
Accurate counting and high-throughput sampling enhance statistical confidence.

Conclusions:

Flow cytometry is the gold standard for critical applications in clinical, research, and environmental fields.
Fluidic systems are key to flow cytometry's analytical and sorting capabilities.
Advancements in fluidics promise further improvements in flow cytometry performance.