Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A specific growth hormone-binding protein in human plasma: initial characterization.

G Baumann, M W Stolar, K Amburn

    The Journal of Clinical Endocrinology and Metabolism
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessing cardiac autonomic function via heart rate variability analysis requires monitoring respiration.

    Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2016
    Same author

    A primary intravascular synovial sarcoma causing deep-vein thrombosis and pulmonary embolism in a 20-year-old woman.

    Current oncology (Toronto, Ont.)·2015
    Same author

    [Will telemonitoring be adopted by patients with chronic heart failure?].

    Deutsche medizinische Wochenschrift (1946)·2014
    Same author

    [Diagnosis of sleep disordered breathing using portable methods].

    Pneumologie (Stuttgart, Germany)·2012
    Same author

    [Sleep-disordered breathing and atrial fibrillation].

    Herzschrittmachertherapie & Elektrophysiologie·2012
    Same author

    [Sleep apnoea syndrome in the rehabilitation setting].

    Herz·2011

    A novel plasma binding protein for human growth hormone (hGH) was identified, explaining the presence of large molecular weight hGH in circulation. This specific binding protein has a high affinity and low capacity, influencing circulating hGH levels.

    Area of Science:

    • Endocrinology
    • Protein Chemistry
    • Biochemistry

    Background:

    • Human growth hormone (hGH) circulates in plasma as various size isomers, including oligomers.
    • The presence of large molecular weight hGH in plasma, disproportionately high compared to the pituitary, is not fully understood.

    Purpose of the Study:

    • To investigate if binding to plasma proteins contributes to the formation of large molecular weight hGH.
    • To characterize the binding interaction between hGH and potential plasma binding components.

    Main Methods:

    • Incubation of radiolabeled monomeric hGH with human plasma/serum.
    • Analysis of plasma hGH patterns using Sephadex G-100 and G-200 chromatography.
    • Saturation/Scatchard analysis to determine binding kinetics and capacity.

    Related Experiment Videos

    Main Results:

    • A component with an apparent molecular weight of 85,000 was detected, indicating hGH binding to plasma proteins.
    • Binding was specific, rapid, reversible, and inhibited by unlabeled hGH (association constant of 2-3 X 10(8) M-1).
    • A heat-labile binding protein (60,000-65,000 MW) was identified, binding one hGH molecule to form an 80,000-85,000 MW complex, accounting for at least 15-18% of circulating hGH.

    Conclusions:

    • A specific, high-affinity, low-capacity binding protein for hGH exists in human plasma.
    • This binding protein contributes to the formation of "big-big" hGH, a large molecular weight form.
    • The biological significance of this hGH binding protein requires further investigation.