Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex.

Neuron·2026
Same author

A Modular Bioadhesive Incorporating Mussel Adhesive Protein and EGF for Robust Growth Factor Immobilization and Enhanced Wound Healing.

Biomacromolecules·2026
Same author

Structure basis for the activation of KCNQ2 by endogenous and exogenous ligands.

Cell reports·2025
Same author

Structure-guided screening identifies Tucatinib as dual inhibitor for MCT1/2.

EMBO reports·2025
Same author

Elucidation of the zinc binding site in KCNQ channels.

British journal of pharmacology·2025
Same author

Peripheral Blood Exosomal miR-184-3p in Methamphetamine Use Disorder: Biomarker Potential and CRTC1-Mediated Neuroadaptation.

Current issues in molecular biology·2025

Related Experiment Video

Updated: May 13, 2026

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.3K

A user-friendly fluorescent biosensor for precise lactate detection and quantification in vitro.

Qiwei Wang1, Sai Shi1, Si Liu1

  • 1Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, P. R. China. sye@tju.edu.cn.

Chemical Communications (Cambridge, England)
|October 15, 2024
PubMed
Summary
This summary is machine-generated.

We developed LaconicSF, a novel biosensor for accurate lactate quantification. This tool offers high specificity for detecting lactate in cell culture, improving metabolite analysis.

More Related Videos

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.0K
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
10:17

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

6.4K

Related Experiment Videos

Last Updated: May 13, 2026

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.3K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.0K
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
10:17

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

6.4K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Metabolomics

Background:

  • Lactate is a critical metabolite in biological systems.
  • Standardization of lactate quantification methods is essential for accurate analysis.
  • Existing methods may lack specificity or user-friendliness.

Purpose of the Study:

  • To develop a novel lactate-responsive biosensor named LaconicSF.
  • To achieve highly specific lactate detection.
  • To enable efficient lactate quantification in cell culture media.

Main Methods:

  • Development of LaconicSF, a lactate-specific biosensor.
  • Testing and validation of LaconicSF in Chinese Hamster Ovary (CHO) cell culture medium.
  • Evaluation of biosensor specificity and efficiency for lactate detection.

Main Results:

  • LaconicSF demonstrated exceptional specificity in lactate detection.
  • The biosensor enabled efficient quantification of lactate in CHO cell culture.
  • LaconicSF proved to be a user-friendly detection tool.

Conclusions:

  • LaconicSF is a highly specific and efficient biosensor for lactate quantification.
  • The developed biosensor is suitable for analyzing lactate in CHO cell culture.
  • LaconicSF shows potential for broader in vitro lactate detection applications.