Related Experiment Videos
Protein method influences on calculation of tissue receptor concentration
American Journal of Clinical Pathology
|January 1, 1986
Summary
The Lowry protein method, often a reference, is imprecise and time-consuming for tissue receptor concentration. Automated turbidometric or Coomassie dyebinding methods offer better agreement for accurate protein standardization.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Molecular Biology
Background:
- Accurate estimation of tissue receptor concentration is crucial in biological and medical research.
- Various protein quantification methods are employed, but their comparative performance is not well-established.
- The choice of protein assay can significantly influence the calculated receptor concentrations.
Purpose of the Study:
- To compare the performance, analytic variability, and accuracy of six common protein quantification methods.
- To evaluate the impact of different protein standardization techniques on tissue receptor concentration measurements.
- To identify the most reliable protein assay for determining receptor concentrations in biological samples.
Main Methods:
- Six different protein quantification methods were assessed.
- Performance metrics including precision, variability, and accuracy were evaluated using bovine serum albumin (BSA) and other protein standards.
- Protein concentrations were determined in cytosols from 46 tumor biopsies using the Lowry method and compared with other assays.
Main Results:
- The Lowry protein procedure, standardized with BSA, was found to be the most imprecise and time-consuming method.
- Assays using BSA standards with other methods yielded results ranging from 74% to 141% of expected values.
- Best agreement with the Lowry method for tumor biopsy cytosols was observed with automated turbidometric (TCA) or Coomassie dyebinding procedures.
Conclusions:
- The selection of a protein quantification method and its standardization significantly impacts the accuracy of tissue receptor concentration calculations.
- The Lowry method, despite its common use, demonstrates considerable variability and is less accurate compared to other methods like automated turbidometric or Coomassie dyebinding.
- Improved protein standardization, particularly for spectrophotometric methods, can reduce the overestimation of receptor protein concentrations.