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Published on: October 6, 2022
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Fluorescence detection of adenosine triphosphate based on dimeric G-quadruplex.
Qiangsheng Xiao1, Yangfan Chen2, Xiao Yu1
1Department of Hepatobiliary and Pancreatic Surgery, The Third Xiangya Hospital of Central South University, Changsha, China.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 15, 2024
Summary
We developed a new sensor for detecting adenosine triphosphate (ATP), a key energy molecule. This method uses fluorescence and offers a simpler way to measure ATP levels in biological samples.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Adenosine triphosphate (ATP) is a vital energy carrier in biological systems.
- ATP levels are crucial indicators for diseases, cell viability, and food quality.
- Existing ATP detection methods face challenges with material preparation, instrument dependency, and complex procedures.
Purpose of the Study:
- To develop a novel, sensitive, and straightforward sensor for adenosine triphosphate (ATP) detection.
- To overcome the limitations of current ATP detection methodologies.
Main Methods:
- A fluorescence-based sensor utilizing the interaction between dimeric G-quadruplex (Di-G4) and thioflavin T (ThT).
- The sensor relies on the inhibitory effect of ATP on S1 nuclease-mediated cleavage of Di-G4, leading to fluorescence recovery.
- Optimized reaction conditions for fluorescence enhancement and ATP quantification.
Main Results:
- A linear correlation between fluorescence intensity and ATP concentrations was established in the range of 0.5–120 µM.
- The sensor achieved a low detection limit of 245 nM for ATP.
- The method demonstrated satisfactory performance in measuring ATP content in apple samples, comparable to commercial assay kits.
Conclusions:
- The proposed Di-G4/ThT fluorescence sensor offers a sensitive and efficient approach for ATP detection.
- This method provides a simplified alternative to existing ATP detection techniques, with potential applications in various biological and food quality assessments.
