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Updated: Jun 17, 2026

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Fluorescence detection strategy based on DNA tetrahedron and ATP-assisted signal amplification for intracellular
Yangfan Chen1, Xi Zhou1, Fangzhi Chen2
1School of Life Sciences, Central South University, Changsha 410013, Hunan, China.
Abstract:
Hypochlorous acid (HClO) serves as a vital reactive oxygen species within the immune system, facilitating defense against microbial invasion and maintaining immune homeostasis. However, excessive accumulation of HClO exerts adverse effects on the body and acts as a critical driver of various neuroinflammatory and cardiovascular diseases. Herein, an detection strategy based on DNA tetrahedron nanostructure and ATP-assisted signal amplification was used for sensitive HClO analysis. The developed system exhibited a linear range of 0.01-3 μM, with a detection limit of 7.49 nM. The assay completed within 30 min, enabling efficient analysis of HClO. Finally, the proposed method was applied to fluorescence imaging of HClO in RAW 264.7 cells, showcasing its potential for intracellular analysis.
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