Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Specific 125I radioimmunoassay for morphine.

D J Edwards, Z Popovski, T J Baumann

Clinical Chemistry

|January 1, 1986

Summary

This study details a radioimmunoassay for morphine, effectively removing its main metabolite. The method offers accurate quantification for morphine concentrations between 10-100 µg/L with high precision.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Response: Use of the WISCI-II score in assessing outcome of intensive robot-assisted gait training in spinal cord injury.

Spinal cord·2024

Same author

Reverse supply chain practices for construction and demolition waste in the Brazilian Amazon: a multi-stakeholder view.

Journal of material cycles and waste management·2023

Same author

Investigation of interfacial strength in nacre-mimicking tungsten heavy alloys for nuclear fusion applications.

Scientific reports·2023

Same author

A new mechanism for void-cascade interaction from nondestructive depth-resolved atomic-scale measurements of ion irradiation-induced defects in Fe.

Science advances·2020

Same author

The corticomotor projection to liminally-contractable forearm muscles in chronic spinal cord injury: a transcranial magnetic stimulation study.

Spinal cord·2016

Same author

A Halo-Shaped Electrode Holder System for HD-tDCS is a Practical and Flexible Alternative to the EEG Cap 4×1-Ring Montage.

Brain stimulation·2015

Area of Science:

Pharmacology
Analytical Chemistry
Clinical Diagnostics

Background:

Morphine is a critical opioid analgesic with a narrow therapeutic index.
Accurate quantification of morphine is essential for therapeutic drug monitoring and forensic analysis.
Existing methods for morphine quantification may lack specificity or require complex procedures.

Purpose of the Study:

To develop and validate a rapid, specific, and sensitive radioimmunoassay (RIA) for morphine quantification.
To assess the method's performance in terms of accuracy, precision, and sensitivity.
To evaluate the suitability of the RIA for routine laboratory use.

Main Methods:

Samples undergo an extraction procedure to remove morphine-3-glucuronide, the primary metabolite.
Quantification is performed using a commercial radioimmunoassay kit with 125I-labeled morphine.
Assay validation included assessment of within-run and between-run coefficients of variation (CV).

Main Results:

The radioimmunoassay accurately measures morphine concentrations in the range of 10 to 100 micrograms/L.
High precision was demonstrated with a within-run CV of less than 2.0% and a between-run CV of 3.5%.
The method is rapid, specific, and sensitive, requiring minimal technical skills and common laboratory equipment.

Conclusions:

The developed radioimmunoassay provides a reliable and efficient method for morphine quantification.
This assay is suitable for routine clinical and forensic laboratories due to its simplicity and performance.
The method's specificity in removing morphine-3-glucuronide enhances the accuracy of morphine measurements.

Related Experiment Videos