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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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27-Hydroxycholesterol Negatively Affects the Function of Bone Marrow Endothelial Cells in the Bone Marrow
Soo-Yeon Woo1, Wan-Seog Shim1, Hyejin Lee1
1Department of Convergence Medicine, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
International Journal of Molecular Sciences
|October 16, 2024
Summary
Exogenous 27-hydroxycholesterol (27HC) negatively impacts bone marrow endothelial cells (BMECs), crucial for hematopoietic stem cell (HSC) survival. This study reveals 27HC disrupts BMEC function, affecting HSC regulation.
Area of Science:
- Hematology
- Cell Biology
- Biochemistry
Background:
- Hematopoietic stem cells (HSCs) rely on specific microenvironments for regulation.
- Bone marrow endothelial cells (BMECs) are key regulators of HSCs during hematological stress.
- Previous work linked exogenous 27-hydroxycholesterol (27HC) to HSC and progenitor cell reduction via reactive oxygen species.
Purpose of the Study:
- To investigate the effect of 27HC on BMECs.
- To clarify the role of BMECs in 27HC-mediated HSC regulation.
Main Methods:
- Isolation of CD31+ BMECs and CD31- cells using magnetic-activated cell sorting.
- Treatment of BMECs and HSCs with exogenous 27HC.
- Assessment of BMEC number and adhesion molecule expression.
Main Results:
- Exogenous 27HC treatment decreased the number of BMECs.
- 27HC reduced the expression of adhesion molecules on BMECs.
- BMECs were found to be sensitive to 27HC, impacting HSC maintenance.
Conclusions:
- BMECs are significantly affected by 27HC.
- 27HC-induced alterations in BMECs compromise HSC survival.
- BMECs play a critical role in maintaining HSC populations under 27HC influence.
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