Related Experiment Video
Updated: Jun 12, 2025
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
Published on: May 10, 2020
Protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion
Xun Shi1, Yafei Huang1, Bingying Zhou2
1State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College, 167 North Lishi Road, Xicheng District, Beijing 100037, China.
Abstract:
Human primary cardiomyocytes are a valuable in vitro model for studying human heart physiology and disease. Here, we present a protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion solution. We describe steps for sample collection, tissue pre-processing, and cell dissociation. We then detail procedures for calcium reintroduction and viability assessment. This protocol can significantly improve the efficiency of cardiomyocyte isolation and subsequent cell function, making them an even more reliable model for cardiac research. For complete details on the use and execution of this protocol, please refer to Shi et al.1.
