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Updated: Jun 25, 2026
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
TCRcost: a deep learning model utilizing TCR 3D structure for enhanced of TCR-peptide binding
Fan Li1,2, Xinyang Qian1,2, Xiaoyan Zhu1,2
1School of Computer Science and Technology, Xi'an Jiaotong University, Xi'an, China.
Introduction:
Predicting TCR-peptide binding is a complex and significant computational problem in systems immunology. During the past decade, a series of computational methods have been developed for better predicting TCR-peptide binding from amino acid sequences. However, the performance of sequence-based methods appears to have hit a bottleneck. Considering the 3D structures of TCR-peptide complexes, which provide much more information, could potentially lead to better prediction outcomes.
Methods:
In this study, we developed TCRcost, a deep learning method, to predict TCR-peptide binding by incorporating 3D structures. TCRcost overcomes two significant challenges: acquiring a sufficient number of high-quality TCR-peptide structures and effectively extracting information from these structures for binding prediction. TCRcost corrects TCR 3D structures generated by protein structure tools, significantly extending the available datasets. The main and side chains of a TCR structure are separately corrected using a long short-term memory (LSTM) model. This approach prevents interference between the chains and accurately extracts interactions among both adjacent and global atoms. A 3D convolutional neural network (CNN) is designed to extract the atomic features relevant to TCR-peptide binding. The spatial features extracted by the 3DCNN are then processed through a fully connected layer to estimate the probability of TCR-peptide binding.
Results:
Test results demonstrated that predicting TCR-peptide binding from 3D TCR structures is both efficient and highly accurate with an average accuracy of 0.974 on precise structures. Furthermore, the average accuracy on corrected structures was 0.762, significantly higher than the average accuracy of 0.375 on uncorrected original structures. Additionally, the average root mean square distance (RMSD) to precise structures was significantly reduced from 12.753 Å for predicted structures to 8.785 Å for corrected structures.
Discussion:
Thus, utilizing structural information of TCR-peptide complexes is a promising approach to improve the accuracy of binding predictions.
