Implantable Fluorogenic DNA Biosensor for Stress Detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Implantable sensors are crucial for monitoring analytes linked to cognitive and physiological states.
• Dehydroepiandrosterone sulfate (DHEA-S) is a key biomarker associated with stress levels.
• Developing stable and sensitive implantable biosensors remains a significant challenge.

Purpose of the Study:

• To develop a novel implantable biosensor for detecting dehydroepiandrosterone sulfate (DHEA-S).
• To enhance sensor sensitivity and biostability for in vivo applications.
• To create a platform for monitoring complex biomarkers related to health.

Main Methods:

• Utilized forced intercalation (FIT) aptamers for detecting subtle intramolecular changes during target binding.
• Incorporated a steroid-specific fluorogenic aptamer into a polyethylenimine-based hydrogel.
• Developed an optically transparent hydrogel with optimal properties for biosensing.

Main Results:

• The FIT biosensor fiber demonstrated improved sensitivity and biostability.
• In vitro studies confirmed successful activation in human serum and skin analogue.
• The biosensor exhibited a linear response to physiological concentrations of DHEA-S.

Conclusions:

• The developed implantable FIT biosensor fiber is effective for detecting DHEA-S.
• The polyethylenimine hydrogel provides a suitable matrix for optical biosensors.
• This platform holds potential for monitoring various biomarkers relevant to physiological and psychological health.