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Updated: Jun 10, 2025

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

Implantable Fluorogenic DNA Biosensor for Stress Detection.

Irina Drachuk^1,2, Namrata Ramani³, Svetlana Harbaugh¹

¹711th Human Performance Wing, Human Effectiveness Directorate, AFRL, 2510 Fifth Street, Wright-Patterson AFB, Ohio 45433, United States.

ACS Applied Materials & Interfaces

|October 17, 2024

Summary

This summary is machine-generated.

Researchers developed an implantable biosensor using forced intercalation (FIT) aptamers to detect dehydroepiandrosterone sulfate (DHEA-S), a stress biomarker. This novel sensor shows promise for monitoring physiological and psychological health indicators in vivo.

Keywords:

FIT biosensor fluorogenic aptamer forced-intercalation hydrogel stress monitoring

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Materials Science

Background:

Implantable sensors are crucial for monitoring analytes linked to cognitive and physiological states.
Dehydroepiandrosterone sulfate (DHEA-S) is a key biomarker associated with stress levels.
Developing stable and sensitive implantable biosensors remains a significant challenge.

Purpose of the Study:

To develop a novel implantable biosensor for detecting dehydroepiandrosterone sulfate (DHEA-S).
To enhance sensor sensitivity and biostability for in vivo applications.
To create a platform for monitoring complex biomarkers related to health.

Main Methods:

Utilized forced intercalation (FIT) aptamers for detecting subtle intramolecular changes during target binding.
Incorporated a steroid-specific fluorogenic aptamer into a polyethylenimine-based hydrogel.
Developed an optically transparent hydrogel with optimal properties for biosensing.

Main Results:

The FIT biosensor fiber demonstrated improved sensitivity and biostability.
In vitro studies confirmed successful activation in human serum and skin analogue.
The biosensor exhibited a linear response to physiological concentrations of DHEA-S.

Conclusions:

The developed implantable FIT biosensor fiber is effective for detecting DHEA-S.
The polyethylenimine hydrogel provides a suitable matrix for optical biosensors.
This platform holds potential for monitoring various biomarkers relevant to physiological and psychological health.