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Updated: Jun 12, 2025
Detection of Neuritic Plaques in Alzheimer's Disease Mouse Model
Published on: July 26, 2011
Protocol for detection of glial complement expression in relation to amyloid plaques in mouse brain with combined
Sadhna Rao1, Joanna Yung1, Melissa Gonzalez Edick1
1Genentech, Inc., South San Francisco, CA 94080, USA.
Abstract:
Complement proteins contribute to neurodegeneration in Alzheimer's disease (AD) and are secreted by glia surrounding beta-amyloid (Aβ) plaques. We present an optimized protocol for Aβ plaque detection with tyramide-digoxigenin signal amplification. This is combined with a multiplex mRNA fluorescence in situ hybridization (FISH) panel to assay glial-specific complement expression proximal to Aβ plaques in TauPS2APP mice. We describe steps for tissue preparation and mRNA detection. We then detail steps for the detection of Aβ plaques, image acquisition, and analysis.
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