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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
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H Antigen expression modulates epidermal Keratinocyte Integrity and differentiation.

Seon-Pil Jin1,2,3, Jang-Hee Oh1,2, Namjoo Kaylee Kim1,2

  • 1Department of Dermatology, Seoul National University Hospital, Seoul, Republic of Korea.

Biological Research
|October 18, 2024
PubMed
Summary

Altering H2 antigen expression via FUT1 impacts skin cell adhesion and differentiation. This suggests a role for H2 antigen in skin integrity and diseases like psoriasis.

Keywords:
Blood group antigenCell-cell adhesionDesmogleinDesmosomeEGFREpidermal growth factor receptorFUT1Glycosylationfucosyltransferase1

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Area of Science:

  • Glycobiology
  • Dermatology
  • Cell Biology

Background:

  • ABO blood group antigens (ABH antigens) are crucial for cell surface glycosylation.
  • Reduced ABH antigen expression is observed in inflammatory skin conditions like psoriasis and atopic dermatitis.
  • H antigen, a precursor for A and B antigens, is synthesized by fucosyltransferase 1 (FUT1).

Purpose of the Study:

  • To investigate the impact of H antigens on desmosomes in keratinocytes.
  • To explore the role of FUT1 in regulating skin cell adhesion and differentiation.

Main Methods:

  • Primary human keratinocytes were manipulated for FUT1 expression (siRNA knockdown or overexpression).
  • Cell adhesion, desmosome characteristics, and keratinocyte differentiation markers were analyzed.
  • Epidermal growth factor receptor (EGFR) signaling pathways were investigated.

Main Results:

  • FUT1 knockdown increased cell-cell adhesion and desmosome size, upregulating desmosomal proteins.
  • Reduced H2 antigen expression via FUT1 knockdown enhanced keratinocyte differentiation.
  • FUT1 knockdown decreased EGFR signaling by lowering EGF ligand levels.

Conclusions:

  • FUT1-mediated H2 antigen expression influences keratinocyte cell-cell adhesion and differentiation.
  • Regulation of desmosome composition and EGFR ligand expression by FUT1 is implicated.
  • Altered H2 antigen expression may contribute to the pathogenesis of skin diseases like psoriasis and atopic dermatitis.