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A gold nanomaterial-integrated distance-based analytical device for uric acid quantification in human urine samples
Tapparath Leelasattarathkul1, Thithawat Trakoolwilaiwan1,2, Kawin Khachornsakkul1,3
1Division of Chemistry, Department of Science, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, 10120 Thailand. Kawin.khachornsakkul@tufts.edu.
The Analyst
|October 18, 2024
Summary
We developed a novel paper analytical device (dPAD) using gold nanoparticles for easy, low-cost uric acid quantification in urine. This instrument-free sensor offers rapid disease diagnosis and prognosis.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biomedical Engineering
Background:
- Uric acid quantification is crucial for diagnosing diseases like gout and kidney issues.
- Existing methods often require complex instrumentation and skilled personnel.
- There is a need for cost-effective, user-friendly point-of-care diagnostic tools.
Purpose of the Study:
- To demonstrate the first distance-based paper analytical device (dPAD) for uric acid detection.
- To develop an instrument-free, user-friendly method for rapid uric acid quantification in human urine.
- To utilize gold nanoparticles (AuNPs) as a peroxidase-like nanozyme, replacing horseradish peroxidase (HRP).
Main Methods:
- A dPAD was designed utilizing gold nanoparticles (AuNPs) as a nanozyme.
- Uric acid was quantified via uricase oxidation and AuNP-catalyzed reaction with DAB, producing a colored distance signal.
- The distance of the color change correlated with uric acid concentration.
Main Results:
- The dPAD showed a linear range of 0.50–6.0 mmol L⁻¹ with R² = 0.9922 and a detection limit of 0.25 mmol L⁻¹.
- The assay demonstrated high selectivity, accuracy (99.37–100.35% recovery), and precision (RSD ≤ 4.05%).
- Results were comparable to a commercial uric acid sensor.
Conclusions:
- The developed dPAD provides a cost-effective, simple, and stable method for uric acid quantification.
- This user-friendly device enables rapid, instrument-free diagnosis and prognosis of related diseases.
- The dPAD concept is adaptable for monitoring other biomarkers as a point-of-care device.
