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Characterization of the complete chloroplast genome of Liparis gigantea (Orchidaceae).

Xingyou Jiang1,2,3, Wenting Yang1,2,3, Caixia Peng1,2

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Summary
This summary is machine-generated.

The complete chloroplast genome of the medicinal herb Liparis gigantea was sequenced, revealing its phylogenetic position. This genomic data aids future research on this rare orchid species.

Keywords:
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Area of Science:

  • Plant genomics
  • Orchidaceae family research
  • Medicinal herb genomics

Background:

  • Liparis gigantea is a rare Chinese medicinal herb from the Orchidaceae family.
  • It is known for its distinctively large flowers.
  • Genomic resources for L. gigantea are currently limited.

Purpose of the Study:

  • To sequence and assemble the complete chloroplast (cp) genome of Liparis gigantea.
  • To elucidate the phylogenetic status of L. gigantea within its genus.
  • To provide essential genomic data for future applications of this medicinal herb.

Main Methods:

  • Whole genome next-generation sequencing was employed for cp genome sequencing.
  • Bioinformatic tools were used for genome assembly and gene annotation.
  • Phylogenetic analysis was conducted using the sequenced cp genome data.

Main Results:

  • The complete cp genome of L. gigantea was successfully sequenced and assembled, measuring 158,462 bp with a GC content of 36.9%.
  • The genome features a typical quadripartite structure with large single-copy (LSC), small single-copy (SSC), and inverted repeat (IR) regions.
  • A total of 133 genes were annotated, including 87 protein-coding genes, 38 tRNA genes, and 8 rRNA genes.
  • Phylogenetic analysis confirmed L. gigantea as the sister species to Liparis nervosa and Liparis vivipara.

Conclusions:

  • This study presents the first complete chloroplast genome sequence for Liparis gigantea.
  • The genomic data clarifies the phylogenetic relationships of L. gigantea within the Liparis genus.
  • The findings provide a valuable genomic foundation for the conservation and utilization of this important medicinal herb.