Characterization of the complete chloroplast genome of Liparis gigantea (Orchidaceae)
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View abstract on PubMed
Summary
This summary is machine-generated.The complete chloroplast genome of the medicinal herb Liparis gigantea was sequenced, revealing its phylogenetic position. This genomic data aids future research on this rare orchid species.
Area Of Science
- Plant genomics
- Orchidaceae family research
- Medicinal herb genomics
Background
- Liparis gigantea is a rare Chinese medicinal herb from the Orchidaceae family.
- It is known for its distinctively large flowers.
- Genomic resources for L. gigantea are currently limited.
Purpose Of The Study
- To sequence and assemble the complete chloroplast (cp) genome of Liparis gigantea.
- To elucidate the phylogenetic status of L. gigantea within its genus.
- To provide essential genomic data for future applications of this medicinal herb.
Main Methods
- Whole genome next-generation sequencing was employed for cp genome sequencing.
- Bioinformatic tools were used for genome assembly and gene annotation.
- Phylogenetic analysis was conducted using the sequenced cp genome data.
Main Results
- The complete cp genome of L. gigantea was successfully sequenced and assembled, measuring 158,462 bp with a GC content of 36.9%.
- The genome features a typical quadripartite structure with large single-copy (LSC), small single-copy (SSC), and inverted repeat (IR) regions.
- A total of 133 genes were annotated, including 87 protein-coding genes, 38 tRNA genes, and 8 rRNA genes.
- Phylogenetic analysis confirmed L. gigantea as the sister species to Liparis nervosa and Liparis vivipara.
Conclusions
- This study presents the first complete chloroplast genome sequence for Liparis gigantea.
- The genomic data clarifies the phylogenetic relationships of L. gigantea within the Liparis genus.
- The findings provide a valuable genomic foundation for the conservation and utilization of this important medicinal herb.
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