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Efficient, gigapixel-scale, aberration-free whole slide scanner using angular ptychographic imaging with closed-form

Shi Zhao1, Haowen Zhou1, Siyu Steven Lin1

  • 1Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Biomedical Optics Express
|October 18, 2024
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Summary

A new whole slide imaging system (WSI-APIC) uses angular ptychography for efficient, aberration-free, gigapixel-resolution imaging of pathology slides. This technology enhances pathological analysis and clinical diagnosis with high-quality, detailed visualization.

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Area of Science:

  • Microscopy and Imaging Technologies
  • Pathology and Diagnostic Medicine
  • Computational Imaging

Background:

  • Whole slide imaging (WSI) is crucial for pathological analysis and clinical diagnosis, requiring high-resolution, aberration-free imaging.
  • Existing WSI systems face limitations in spatial bandwidth, aberration control, and scanning efficiency.
  • Detailed visualization of cellular and tissue structures is essential for patient care and treatment planning.

Purpose of the Study:

  • To develop an efficient, large-field-of-view, aberration-free whole slide imaging system.
  • To enhance pathological analysis through high-quality digital imaging of microscopic slides.
  • To overcome limitations of current WSI technologies for improved diagnostic accuracy.

Main Methods:

  • Angular ptychographic imaging with a closed-form solution (WSI-APIC) was employed for image reconstruction.
  • Oblique incoherent illumination was utilized for efficient background segmentation and reduced scanning.
  • A GPU-accelerated algorithm performed digital aberration correction and phase image reconstruction.
  • Scale-invariant feature transform (SIFT) enabled auto-stitching for seamless image concatenation.

Main Results:

  • The WSI-APIC system achieved an optical resolution of 772 nm with a 10×/0.25 NA objective lens.
  • The system captured 80-gigapixel aberration-free phase images of standard microscopic slides.
  • Image acquisition efficiency was enhanced by bypassing background regions.
  • Effective digital aberration corrections and improved optical resolutions were demonstrated.

Conclusions:

  • WSI-APIC offers an efficient solution for generating large-FOV, aberration-free, gigapixel-resolution phase images.
  • The developed system significantly advances pathological imaging capabilities for enhanced diagnosis.
  • This technology holds promise for improving patient care through detailed visualization of tissue samples.