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Surface-Enhanced, Low-Frequency Raman Spectroscopy: A Sensitive Screening Tool for Structural Characterization of
Ka Rlis Be Rziņš1, Ben J Boyd1,2
1Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2100, Denmark.
Analytical Chemistry
|October 18, 2024
Summary
Surface-enhanced, low-frequency Raman spectroscopy (SELFRS) offers a powerful method for pharmaceutical analysis. This technique effectively screens drug crystallization and polymorphic forms, aiding in understanding crystallization processes.
Area of Science:
- Analytical Chemistry
- Materials Science
- Pharmaceutical Sciences
Background:
- Raman spectroscopy is crucial for analyzing molecular structures.
- Surface-enhanced Raman spectroscopy (SERS) enhances sensitivity.
- Low-frequency Raman spectroscopy (LFRS) probes lattice vibrations, important for solid-state characterization.
Purpose of the Study:
- To evaluate Surface-enhanced, low-frequency Raman spectroscopy (SELFRS) as a structural screening tool for pharmaceutical applications.
- To investigate the crystallization behavior of paracetamol with and without benzoic acid using SELFRS.
- To explore the potential of LFR spectral range for multicomponent analysis and understanding crystallization mechanisms.
Main Methods:
- Utilized commercial silver-based SERS substrates for paracetamol crystallization studies.
- Employed two LFR-enabled Raman imaging instruments with 532 and 785 nm lasers.
- Applied chemometric analysis to compare SELFRS, SERS, and combined spectral ranges.
Main Results:
- Both LFR setups differentiated between paracetamol polymorphic forms I and II.
- SELFRS, individually or combined with the fingerprint region, improved surface-enhanced measurements.
- SELFRS provided mechanistic insights into template-assisted crystallization of metastable form II.
Conclusions:
- SELFRS is a valuable tool for pharmaceutical structural screening and multicomponent analysis.
- The LFR spectral range enhances SERS measurements, offering improved differentiation of polymorphic forms.
- SELFRS aids in understanding the mechanisms of crystallization additives in pharmaceutical formulations.
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