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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jun 12, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Protocol for generating high-fidelity proteomic profiles using DROPPS.

Matthew Waas¹, Meinusha Govindarajan², Amanda Khoo²

¹Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.

|October 18, 2024

Summary

This summary is machine-generated.

We developed droplet-based one-pot preparation for proteomic samples (DROPPS), a new low-input method for high-fidelity proteomic profiling of rare cells. This accessible platform analyzes 100-2,500 cells, advancing rare cell population research.

Keywords:

cell biology flow cytometry proteomics

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Last Updated: Jun 12, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Published on: November 15, 2017

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Published on: October 15, 2019

Area of Science:

Proteomics
Cell Biology
Biochemistry

Background:

Proteomic profiling of rare cell populations is challenging due to high sample input requirements.
Existing methods limit the analysis of limited cell numbers, hindering research on scarce biological samples.

Purpose of the Study:

To present a novel, accessible, low-input protocol for high-fidelity proteomic profiling.
To enable deep proteomic analysis of rare cell populations using mass spectrometry.

Main Methods:

Development of droplet-based one-pot preparation for proteomic samples (DROPPS).
Protocol involves depositing cellular material, cell lysis, and protein digestion within a single microliter-droplet well.
Utilizes mass spectrometry for high-fidelity proteomic profiling.

Main Results:

DROPPS platform successfully generates high-fidelity proteomic profiles from as few as 100 cells.
The protocol is designed for low-input sample requirements, accommodating 100-2,500 cells.
Demonstrates the feasibility of deep proteomic analysis on rare cell populations.

Conclusions:

DROPPS offers an accessible solution for low-input proteomic sample preparation.
This protocol is expected to accelerate research on rare cell populations across various biological fields.
Facilitates advancements in understanding the proteome of limited and rare cell types.