Response of phytoplankton community to dissolved organic matter composition and lake trophic state

Human activities, intensified urbanization and climate changes altered source and quantity of dissolved organic matter (DOM), complicating its interaction with phytoplankton in aquatic ecosystems. However, relationship between DOM and phytoplankton in urban lakes strongly disturbed by human activities was still unclear. Thus, a whole-year sampling campaign was conducted in the Tangxun Lake, China's largest urban lake, to reveal the interaction between DOM and phytoplankton. Results indicated that trophic state in the Tangxun Lake varied from mesotrophic to moderately eutrophic. Parallel factor analysis method combined with excitation-emission matrix fluorescence spectroscopy revealed that DOM in the Tangxun Lake consisted of three components, two protein-like components (C1, C3), and one humic-like component (C2). Protein-like components occupied 80% ± 11% of total CDOM pool, mainly due to urbanization driving DOM to be more protein-like, less humic-like. Besides, DOM in the Tangxun Lake was mainly autochthonous input and more recently formed. Furthermore, a total of 129 phytoplankton species were identified, belonging to 78 genera and 7 phyla. Tangxun Lake's phytoplankton community structure was dominated by the Chlorophyta-Bacillariophyta-Cyanophyta type. The temporal succession of phytoplankton varied significantly. It was found that the abundance of Cryptophyta and Cyanophyta were predominant in the mesotrophic state, while Cyanophyta and Bacillariophyta were prevailing in the eutrophic and middle-eutrophic states. As for the interaction between DOM and phytoplankton, results demonstrated that phytoplankton biomass was significantly positively correlated with a (254), a proxy of DOM abundance. Moreover, phytoplankton abundance and biomass significantly positively correlated with autochthonous and freshly released DOM, indicating that the more autochthonous and freshly released DOM, the higher phytoplankton abundance and biomass. Overall, this study provides profound environmental implications for aquatic ecosystem management, especially those strongly affected by human activities.