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Related Experiment Video

Updated: May 3, 2026

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Light-induced targeting enables proteomics on endogenous condensates.

Choongman Lee¹, Andrea Quintana¹, Ida Suppanz²

¹Max Planck Institute for Immunobiology and Epigenetics, Freiburg 79108, Germany; Department of Biological Physics, Max Planck Institute for Immunobiology and Epigenetics, Freiburg 79108, Germany.

|October 19, 2024

Summary

Studying transient cellular condensates is challenging. A new method, light-induced targeting of endogenous condensates (LiTEC), uses optogenetics to target and study these structures in living cells.

Keywords:

IDR LiTEC composition endogenous condensate intrinsically disordered mass spectrometry optogenetics proteomics targeting transcriptional cluster

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Last Updated: May 3, 2026

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

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Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

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Published on: January 5, 2024

Area of Science:

Cellular biology
Molecular biology
Biochemistry

Background:

Endogenous condensates are dynamic cellular structures crucial for biological processes.
Studying transient condensate components is difficult with traditional proteomics methods.

Purpose of the Study:

To develop a novel method for studying endogenous condensates in living cells.
To enable controlled and reversible targeting of molecules into condensates.

Main Methods:

Developed light-induced targeting of endogenous condensates (LiTEC) using optogenetics.
Identified molecular zip codes for condensate targeting.
Combined LiTEC with proximity-based biotinylation (BioID).

Main Results:

Demonstrated successful light-dependent targeting of cargo into condensates.
Uncovered putative components of transcriptional condensates in mouse embryonic stem cells.

Conclusions:

LiTEC provides a powerful tool for investigating endogenous condensate composition and function.
This approach facilitates genome-wide functional studies of cellular condensates.