Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Silver-Surface-Enhanced Raman Spectra of Berberine: Analyte-Induced Surface Changes, Variable Concentration Correlation, and Excitation Wavelength Dependence.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Characterization of Modified PVDF Membranes Using Fourier Transform Infrared and Raman Microscopy and Infrared Nanoimaging: Challenges and Advantages of Individual Methods.

ACS omega·2024
Same author

Unveiling the Crucial Role of Chemical Enhancement in the SERS Analysis of Amphetamine-Metal Interactions on Gold and Silver Surfaces: Importance of Selective Amplification of the Narrow Interval of Vibrational Modes.

Analytical chemistry·2024
Same author

Laser-Induced Reactions of 4-Aminobenzenthiol Species Adsorbed on Ag, Au, and Cu Plasmonic Structures Followed by SERS Spectroscopy. The Role of Substrate and Excitation Energy - Surface-Complex Photochemistry and Plasmonic Catalysis.

ACS omega·2024
Same author

Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy.

Small methods·2021
Same author

Fast Leaps between Millisecond Confinements Govern Ase1 Diffusion along Microtubules.

Small methods·2021

Related Experiment Video

Updated: Jun 10, 2025

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

387

Surpassing the Diffraction Limit in Label-Free Optical Microscopy.

David Palounek1,2, Milan Vala1, Łukasz Bujak1

  • 1Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberská 1014/57, Prague 8 18200, Czech Republic.

ACS Photonics
|October 21, 2024
PubMed
Summary
This summary is machine-generated.

Emerging label-free super-resolution microscopy offers dynamic, high-resolution imaging of biological systems without fluorescent labels. This approach overcomes limitations of traditional methods, enabling deeper understanding of complex cellular processes.

More Related Videos

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

Related Experiment Videos

Last Updated: Jun 10, 2025

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

387
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

Area of Science:

  • Optical microscopy
  • Biophysics
  • Nanotechnology

Background:

  • Super-resolution optical microscopy significantly advanced nanoscale visualization of biological structures.
  • Fluorescence-based techniques provide high specificity and resolution but are limited by labeling requirements, impacting spatiotemporal resolution and observed interactions.
  • Labeling inherently restricts the scope of dynamic biological process observation.

Purpose of the Study:

  • To explore the potential of emerging label-free imaging methods for super-resolution microscopy.
  • To identify key advancements and challenges in label-free super-resolution techniques.
  • To discuss future directions for label-free super-resolution microscopy in understanding complex biological systems.

Main Methods:

  • Utilizes inherent optical phenomena like elastic and inelastic scattering for signal generation directly from the sample.
  • Leverages advanced optical techniques to surpass the diffraction limit without fluorescent labels.
  • Analyzes signals to measure properties such as molecular mass, orientation, and chemical composition.

Main Results:

  • Label-free methods show potential to overcome speed and resolution limitations of fluorescence microscopy.
  • These techniques can capture dynamic processes at biomolecular timescales and single-molecule resolution.
  • Experimental progress indicates the feasibility of achieving super-resolution without labels.

Conclusions:

  • Label-free super-resolution microscopy offers a path to detailed, dynamic imaging of living cells.
  • It surpasses conventional methods by enabling visualization of biological complexities without the need for labels.
  • This technology promises enhanced understanding of biological systems through innovative optical advancements.