Achieving lysozyme functionalization in PDADMAC-NaPSS saloplastics through salt annealing

Hot-pressed saloplastics are dense and transparent polyelectrolyte complex materials governed by ionic crosslinking. Such plastics have several advantages, for example, salt water processibility and recyclability. Here, we demonstrate a simple but effective post-treatment method to incorporate lysozyme as a biocatalytic component into the hot-pressed saloplastics. Changes in salt concentration can be used for annealing and curing the saloplastics, where the temporary opening allows for lysozyme loading. This process was carefully examined by two different routes and the salt concentrations and incubation times were varied systematically. Optimised saloplastics showed an enzymatic activity against Micrococcus lysodeikticus of 4.44 ± 0.37 U cm^-2 and remained partially active (∼72% activity preserved) after 7 days. This approach opens new routes to incorporate enzymes or other biological functionality into saloplastics which is difficult to achieve for conventional plastics.