Chromosome analysis on a cell line (CE48T/VGH) derived from a human esophageal carcinoma

The cell line CE48T/VGH was established from an epidermoid carcinoma of the middle third of the esophagus of a 58-year-old male patient. Cytogenetic analysis at passages 90-99 showed that the line was hypotetraploid, with a mean chromosome number of 73. None of the 50 karyotyped cells had a normal chromosome #1 or Y. Cells with multiple copies of some of the autosomes were observed frequently. Structural rearrangements were numerous, especially of chromosomes #1, #9, #14, X, and Y.