Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Study of electroreflectance spectrum and Franz-Keldysh effect at metal-GaAs interfaces].

Guang pu xue yu guang pu fen xi = Guang pu·2008

Same author

[Study on electro-degradation of new conjugated polymer PFO-BT15 light emitting diodes].

Guang pu xue yu guang pu fen xi = Guang pu·2008

Same author

Comparison of the curative effects of video assisted thoracoscopic anterior correction and small incision, thoracotomic anterior correction for idiopathic thoracic scoliosis.

Chinese medical journal·2008

Same author

Distribution and sources of mercury in soils from former industrialized urban areas of Beijing, China.

Environmental monitoring and assessment·2008

Same author

[Main flavonoids from Sophora flavescenes].

Yao xue xue bao = Acta pharmaceutica Sinica·2008

Same author

External validation and prediction employing the predictive squared correlation coefficient test set activity mean vs training set activity mean.

Journal of chemical information and modeling·2008

Search research articles

Related Experiment Video

Updated: Jun 9, 2025

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

Two Squaramide-Based Fluorescent Probes for Cu²⁺ and Cd².

Yuanwei Li^1,2, Hongxu Wang^1,2, Qian Tao^1,2

¹College of Chemistry and Chemical Engineering of China West Normal University, Nanchong, 637000, China.

Journal of Fluorescence

|October 21, 2024

Summary

New squaramide ligands detect toxic copper (Cu2+) and cadmium (Cd2+) ions in environmental samples. These probes offer rapid detection across a wide pH range, crucial for environmental monitoring.

Keywords:

Cd2+ detection Cu2+ detection Fluorescent probe Squaramide

More Related Videos

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

Related Experiment Videos

Last Updated: Jun 9, 2025

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

Area of Science:

Environmental Science
Analytical Chemistry
Materials Science

Background:

Toxic metal ion detection is vital for environmental safety.
Developing sensitive and selective probes is an ongoing challenge.
Squaric acid and imine functionalities offer unique recognition properties.

Purpose of the Study:

To synthesize and characterize novel squaramide ligands for toxic metal ion detection.
To evaluate the ligands' ability to recognize copper (Cu2+) and cadmium (Cd2+) ions.
To determine the probes' performance metrics, including limit of detection (LOD) and response time.

Main Methods:

Synthesis of two squaramide ligands (2a, 2b) incorporating squaric acid and imine groups.
Spectroscopic analysis to confirm ligand structure and metal ion binding.
Job's plot, Electrospray Ionization Mass Spectrometry (ESI-MS), and proton nuclear magnetic resonance (1H NMR) to elucidate binding stoichiometry and mode.

Main Results:

Ligands 2a and 2b demonstrated selective recognition of Cu2+ and Cd2+ ions.
Achieved low limits of detection (LOD) for Cu2+ (1.26 × 10-8 M) and Cd2+ (2.04 × 10-8 M).
Probes exhibited fast response times and effectiveness over a broad pH range.

Conclusions:

The developed squaramide ligands are effective tools for detecting toxic Cu2+ and Cd2+ ions.
The probes offer advantages in speed and operational pH for environmental sensing applications.
Further investigation into binding mechanisms confirmed the probe-ion interactions.