Non-electroactive bacteria behave variously in AnMBR biofilm control using electric field

Electroactive bacteria are often regarded as key players responding to electric fields that are used to control biofilm development during AnMBR (anaerobic membrane bioreactor) operation. Consequently, little attention has been given to non-electroactive bacteria in the same systems because of their incapability to acquire and transfer electrons directly. However, in this study, we identified some functionally important non-electroactive bacteria from biofilm established under low-voltage (0, 0.3, 0.5 and 1 V) electric fields in AnMBRs, designated as E-AnMBRs in this study. During the whole experiment, non-electroactive bacteria, mainly belonging to Proteobacteria, Bacteroidetes, and Chloroflexi, were found in all biofilm samples taken from each E-AnMBR. Under 0.3 V and 1 V conditions, non-electroactive bacteria did not seem to contribute to the development of biofilm significantly. Whereas under 0.5 V conditions, the growth of non-electroactive bacteria contributed up to 0.61 kPa/day biofilm formation. Therefore, 0.5 V was identified as a critical voltage, leading to the most severe biofilm formation. The microbial community structure in the reactor with a 0.5 V electric field was distinctly unique, caused by the increase of non-electroactive bacterial activity and the upregulation of their metabolic pathways. Notably, functional genes involved in carbon metabolism and oxidative phosphorylation pathway were upregulated. Furthermore, the 0.5 V electric field enhanced the protein/polysaccharide ratio and increased zeta potential to 31.6 mV (p < 0.01) of the biofilm samples. This was because upregulating quorum sensing genes accelerated the coordinated gene regulations and functional activities among non-electroactive bacteria.