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Updated: Jul 29, 2026
08:39
Murine Colitis Modeling using Dextran Sulfate Sodium DSS
Published on: January 19, 2010
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Host-microbe interaction-mediated resistance to DSS-induced inflammatory enteritis in sheep
Shuo Yan1,2, Ruilin Du1,2, Wenna Yao1,2
1Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Xilinguole Rd. 49, Yuquan District, Hohhot, 010020, China.
Microbiome
|October 21, 2024
Summary
Roseburia bacteria enhance disease resistance by stabilizing the intestinal barrier, offering a potential treatment for colitis. This study highlights the protective role of beneficial microbes in host-microbiota interactions.
Area of Science:
- Microbiome research
- Immunology
- Animal husbandry
Background:
- Disease resistance is linked to immune function and tolerance, impacting animal and human health.
- The role of microbes in host-microbiota interactions for disease resistance is not well understood.
- Investigating microbial mechanisms in intestinal diseases can reveal new therapeutic targets.
Purpose of the Study:
- To uncover the role of microbes in intestinal diseases and their mechanisms of action.
- To identify potential new treatments for intestinal diseases based on microbial interactions.
- To explore host-microbiota interactions in disease-resistant phenotypes.
Main Methods:
- Established a DSS-induced colitis model in two sheep breeds (Hu and East Friesian).
- Conducted multi-omics analyses (metagenomics, metabolomics, transcriptomics) on sheep samples.
- Utilized fecal microbiota transplantation (FMT) in mice, followed by Roseburia bacteria treatment and histological analysis.
Main Results:
- Hu sheep showed increased Roseburia bacteria and bile acid secretion, enhancing disease resistance.
- East Friesian sheep exhibited harmful bacteria enrichment, activating TNFα signaling and worsening injury.
- Roseburia bacteria treatment demonstrated a significant curative effect in FMT mice with colitis.
Conclusions:
- Roseburia bacteria increase beneficial microorganisms and stabilize the intestinal barrier in DSS-treated sheep.
- Roseburia bacteria exhibit a protective effect on the intestinal barrier in Hu sheep.
- Host-microbiota interactions, particularly involving Roseburia, are crucial for IBD progression and treatment.
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