Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of the C4 proteins encoded by okra-infecting geminiviruses in India.

BMC plant biology·2026
Same author

Intra-viral protein-protein interactions could expand the functional portfolio of C4 in okra-infecting geminiviruses.

Virus research·2026
Same author

Machine learning, bioinformatics analysis and chemical screening streamline target validation by identifying RNA helicase as a druggable essential protein in tobacco mosaic virus.

Journal of advanced research·2026
Same author

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.

Nature communications·2026
Same author

FaRIF as a key regulator of strawberry fruit ripening: deciphering its targets and interaction networks.

Horticulture research·2026
Same author

Virus induced gene editing using potyviral vectors in Cas12a expressing plants.

Horticulture research·2026

Related Experiment Video

Updated: Jun 9, 2025

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

49.1K

CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation.

Elena Garcia-Perez1, Marta Vazquez-Vilar1, Rosa Lozano-Duran2

  • 1Instituto de Biología Molecular y Celular de Plantas, CSIC-UPV, Valencia, Spain.

Plant Biotechnology Journal
|October 22, 2024
PubMed
Summary

A new plant-based system, CuBe, uses copper sulfate to activate gene expression for producing biomolecules. This offers a cost-effective and scalable alternative to current methods for recombinant protein manufacturing.

Keywords:
Nicotiana benthamianacopper switchgeminivirusinducible expressionmolecular farmingplant synthetic biology

More Related Videos

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants
05:52

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants

Published on: November 17, 2023

2.4K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.2K

Related Experiment Videos

Last Updated: Jun 9, 2025

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

49.1K
Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants
05:52

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants

Published on: November 17, 2023

2.4K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.2K

Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Biotechnology

Background:

  • Sustainable biomolecule manufacturing demands efficient plant-based systems.
  • Agroinfiltration is standard but costly and variable for large-scale production.

Purpose of the Study:

  • Introduce the CuBe system, a novel copper-activated, replicative expression platform in plants.
  • Evaluate its potential for cost-effective and scalable recombinant protein production.

Main Methods:

  • Developed a synthetic circuit with four genetic modules in *Nicotiana benthamiana*.
  • Utilized a bean yellow dwarf virus (BeYDV)-derived vector for gene of interest (GOI) expression.
  • Activated the system using copper sulfate (CuSO4) to induce replication and protein production.

Main Results:

  • Demonstrated successful expression of enhanced green fluorescent protein (eGFP) and an anti-SARS-CoV-2 antibody in *N. benthamiana*.
  • Achieved high-level protein accumulation upon copper treatment.
  • Showcased system functionality in post-harvest applications for biomanufacturing.

Conclusions:

  • The CuBe system provides a promising, cost-effective, and scalable alternative to agroinfiltration.
  • Copper-inducible activation enables efficient recombinant protein production in plants.
  • Potential for significant impact on large-scale biomanufacturing through plant-based systems.