Recombinant Lactiplantibacilllus plantarum modulate gut microbial diversity and function

Area of Science:

• Microbiology
• Immunology
• Gastroenterology

Background:

• Gut microbes are crucial for host health and disease indication.
• Lactiplantibacilllus plantarum (L. plantarum) is recognized for activating immune responses and its use in mucosal vaccines.

Purpose of the Study:

• To investigate the interaction between recombinant L. plantarum and the gut microbiota.
• To evaluate the immunomodulatory effects of recombinant L. plantarum.

Main Methods:

• Analysis of gut microbial operational taxonomic units (OTUs) and diversity (Shannon-Wiener index).
• Beta diversity analysis to assess changes in microbial structure.
• Measurement of serum IgG, IgG1, and fecal sIgA, along with CD4+ T cell and IgA+ B cell populations.

Main Results:

• Recombinant L. plantarum significantly increased gut bacterial species diversity and altered microbial structure.
• Recombinant NC8 L. plantarum expressing viral P14.5 and IL-33 enhanced gut bacterial metabolism and immune functions.
• Elevated IgG, IgG1, and sIgA levels, along with increased CD4+ T and IgA+ B cells, demonstrated the immunomodulatory role of the gut microbiota mediated by recombinant L. plantarum.

Conclusions:

• Recombinant L. plantarum plays a significant role in modulating gut microbiota and immunity.
• These findings support the therapeutic potential of L. plantarum in various applications.