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Updated: Jun 9, 2025
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Molecular networking-guided isolation of melanogenesis inhibitory dihydro-β-agarofuran sesquiterpenoids from
Jun Gu Kim1, Jae Sang Han1, Yong Beom Cho1
1College of Pharmacy, Chungbuk National University, Cheongju, 28160, Republic of Korea.
Abstract:
Seven previously undescribed dihydro-β-agarofurans (1-7) together with 28 known compounds were isolated from Celastrus orbiculatus fruits using LC-MS/MS-based molecular networking-guided purification. Their structures were elucidated through 1D and 2D-NMR, HRESIMS analysis, and ECD quantum chemical calculations. The inhibitory effects of all isolated compounds on α-MSH-induced melanin production in B16F0 melanoma cells were tested. Compounds 1, 11, 12, 19-21, 23, 29, 31, 34, and 35 showed significant inhibitory effects on melanin production with IC50 values ranging from 11.3 to 30.1 μM.
