Cryptocarya moschata fractions decrease planktonic cells and biofilms of Candida albicans and Streptococcus mutans

Area of Science:

• Phytochemistry
• Microbiology
• Antimicrobial Agents

Background:

• Cryptocarya species extracts exhibit antimicrobial properties.
• Fractionation of extracts can enhance their antimicrobial potential.
• Biofilm formation by Candida albicans and Streptococcus mutans poses significant health challenges.

Purpose of the Study:

• To evaluate the antimicrobial activity of Cryptocarya moschata fractions against planktonic cells and biofilms.
• To determine the efficacy of different fractions against Candida albicans and Streptococcus mutans.

Main Methods:

• Four fractions of C. moschata were prepared: hexane, acetate/hexane (1:1), ethyl acetate, and water.
• Antimicrobial activity was assessed using colony-forming unit counts (CFU/mL) for planktonic cells and biofilms.
• Biofilm inhibition was further evaluated by measuring cell metabolic activity and using confocal laser scanning microscopy (CLSM).

Main Results:

• Acetate/hexane (1:1) and 100% ethyl acetate fractions reduced planktonic Candida albicans.
• No significant effect was observed on planktonic Streptococcus mutans.
• All tested C. moschata fractions reduced biofilm CFU/mL by 2-5 logs and cell metabolic activity by approximately 80%.
• CLSM revealed reduced microorganism viability and extracellular matrix damage in biofilms.

Conclusions:

• Cryptocarya moschata fractions, particularly acetate/hexane (1:1) and 100% ethyl acetate, demonstrate significant antimicrobial activity against biofilms.
• These fractions hold potential for developing novel anti-biofilm agents.