Comparison of Survivin Determination by Surface-Enhanced Fluorescence and Raman Spectroscopy on Nanostructured Silver...

Area of Science:

• Biotechnology
• Analytical Chemistry
• Oncology

Background:

• Survivin is a protein promoting cell proliferation and inhibiting apoptosis.
• Overexpression of survivin is linked to various cancers, making it a key diagnostic and therapeutic marker.
• Sensitive detection methods are crucial for survivin's role in cancer diagnosis and monitoring.

Purpose of the Study:

• To compare surface-enhanced fluorescence and Raman spectroscopy for survivin detection.
• To evaluate nanowire-based substrates decorated with silver nanoparticles (dendrites vs. aggregates) as immunoassay platforms.
• To establish a sensitive and reliable method for survivin quantification in human serum.

Main Methods:

• Development of a two-step non-competitive immunoassay using specific monoclonal antibodies.
• Utilized silver dendrite and aggregate nanostructures on nanowire surfaces as substrates.
• Employed surface-enhanced fluorescence with rhodamine-labeled streptavidin and Raman spectroscopy with peroxidase-labeled streptavidin and TMB substrate.

Main Results:

• Silver dendrite substrates yielded higher fluorescence signals, while silver aggregate substrates showed stronger Raman signals.
• Both fluorescence and Raman spectroscopy methods achieved a detection limit of 12.5 pg/mL.
• A linear dynamic range up to 500 pg/mL was established, enabling survivin determination in human serum.

Conclusions:

• Both surface-enhanced fluorescence and Raman spectroscopy, when optimized with appropriate silver nanostructures, offer sensitive survivin detection.
• The developed methods are suitable for survivin quantification in human serum for ovarian cancer diagnosis and monitoring.
• This work highlights the potential of nanostructured substrates in developing advanced biosensing platforms for clinical applications.