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Related Experiment Video

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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Paper-Based DNA Biosensor for Rapid and Selective Detection of miR-21.

Alexander Hunt1, Sri Ramulu Torati2, Gymama Slaughter1,2

  • 1Center for Bioelectronics, Old Dominion University, Norfolk, VA 23508, USA.

Biosensors
|October 25, 2024
PubMed
Summary

This study presents a novel electrochemical biosensor for early cancer detection using microRNA-21 (miR-21) detection. The cost-effective, paper-based device offers rapid and sensitive diagnostics for low-resource settings.

Keywords:
gold inkjet printinggold nanoparticlesmicroRNA-21 detectionpaper-based biosensor

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cancer Diagnostics

Background:

  • Cancer is a leading global cause of death, emphasizing the need for accessible early detection methods.
  • Healthcare disparities limit access to advanced cancer diagnostics, particularly in resource-limited settings.
  • MicroRNAs (miRs), such as miR-21, are promising biomarkers for early cancer detection due to their differential expression.

Purpose of the Study:

  • To develop a cost-effective, sensitive, and rapid electrochemical paper-based biosensor for the quantitative detection of miR-21.
  • To enable point-of-care cancer diagnostics suitable for low-resource environments.
  • To address healthcare disparities in cancer screening and diagnosis.

Main Methods:

  • Fabrication of an electrochemical paper-based biosensor using gold inkjet printing.
  • Immobilization of single-stranded DNA-21 (ssDNA-21) on gold nanoparticles (AuNPs) via thiolated self-assembled monolayers.
  • Detection of miR-21 through hybridization, leading to changes in electron transfer and current suppression.

Main Results:

  • The biosensor achieved a linear detection range of 1 fM to 1 nM for miR-21.
  • Demonstrated high sensitivity with a detection limit of 0.35 fM in serum.
  • Exhibited a rapid response time of 15 minutes and excellent selectivity against interferents.

Conclusions:

  • The developed gold inkjet-printed electrochemical paper-based biosensor provides a viable tool for sensitive and specific miR-21 detection.
  • This technology holds significant potential for advancing cost-effective, point-of-care cancer diagnostics in underserved regions.
  • The study highlights a promising approach to mitigate healthcare disparities in cancer diagnostics.