Differentiation of Vespa velutina nigrithorax Colonies Using Volatile Organic Compound Profiles of Hornets and Nests

Vespa velutina (Lepeletier, 1836) (Hymenoptera: Vespidae) is a eusocial insect that lives in colonies of hundreds to thousands of individuals, which are divided into castes according to their task: queens, workers, and males. The proper functioning of the colony requires communication between the individuals that make up the colony. Chemical signals (pheromones) are the most common means of communication used by these insects to alarm and differentiate between individuals belonging or not to the colony. In this work, profiles of volatile organic compounds were obtained from the hornets and the external cover of four secondary nests located in the Basque Country. The obtained profiles were treated using chemometric tools. The grouping of hornets and nests according to the different colonies and geographical location was observed. In total, 37 compounds were found in common in hornets and nests. Most of them have been reported in the literature as belonging to different insects and plant species. This would corroborate the transfer of chemical compounds between the nest and the hornets' nest and vice versa. This information could be applied to the development of more efficient control methods for this invasive species, such as attractive traps or baits containing the relevant compounds.