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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jun 9, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Gating Single-Molecule Fluorescence with Electrons.

Katharina Kaiser^1,2, Song Jiang¹, Michelangelo Romeo¹

¹<a href="https://ror.org/00pg6eq24">Université de Strasbourg</a>, CNRS, IPCMS, UMR 7504, F-67000 Strasbourg, France.

Physical Review Letters

|October 25, 2024

Summary

This summary is machine-generated.

Tip-enhanced photoluminescence (TEPL) microscopy reveals how single-molecule fluorescence is quenched by tunneling electrons. This study demonstrates precise control over molecular emission by manipulating tip position and bias voltage.

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Last Updated: Jun 9, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 31, 2015

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Area of Science:

Single-molecule spectroscopy
Scanning probe microscopy
Quantum optics

Background:

Tip-enhanced photoluminescence (TEPL) enables nanoscale optical characterization.
Understanding electron-molecule interactions is crucial for nanoscale optoelectronics.

Purpose of the Study:

To investigate the mechanism of fluorescence quenching in single molecules using TEPL.
To demonstrate control over single-molecule emission via scanning tunneling microscopy (STM).

Main Methods:

Subnanometer spatial resolution TEPL measurements on isolated molecules.
Utilizing a scanning tunneling microscope (STM) for precise tip positioning.
Employing a many-body model and rate equations to analyze luminescence.

Main Results:

Observed progressive fluorescence quenching as tip-molecule distance decreased.
Identified population inversion between molecular states (S0 and D0-) as the quenching mechanism.
Demonstrated gating of molecular emission using bias voltage and tip position.

Conclusions:

TEPL provides insights into electron-induced quenching in single molecules.
Precise control over molecular fluorescence is achievable through STM manipulation.
The demonstrated approach is broadly applicable to various molecular systems.