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Published on: January 19, 2024
Determining the Effect of Non-Thermal Plasma on the Transmembrane Kinetics of Melittin through Molecular Explorations
Yanxiu Cui1, Tong Zhao1, Yanxiong Niu1
1School of Electrical Engineering, Shandong University, Ji'nan 250061, China.
Abstract:
Non-thermal plasma (NTP) synergistic anticancer strategies are a current hotspot of interest at the intersection of plasma biomedicine. Melittin (MEL) has been shown to inhibit cancer in many malignant tumors; however, its clinical application is controversial. Therefore, the transmembrane process and mechanism of MEL activity in different cell systems were studied and the combination of MEL and NTP was proposed in this paper. The results showed that the electrostatic attraction between MEL and the lipid bilayer contributes to the stable orientation of MEL on the membrane surface. In addition, sialic acid overexpression affects the degree to which MEL binds the membrane system and the stability of the membrane structure. The use of NTP to reduce the dosage of MEL and its related nonspecific cytolysis activity has certain clinical application value. The results of this study provide theoretical support for improving the clinical applicability of MEL and contribute to the further development of plasma biomedicine.
Insights
Non-thermal plasma (NTP) enhances melittin (MEL) anticancer effects by improving its membrane binding and reducing side effects. This combination shows promise for clinical applications in plasma biomedicine.
Area of Science:
- Plasma biomedicine
- Biochemistry
- Cancer research
Background:
- Melittin (MEL) exhibits anticancer properties but faces clinical limitations due to its controversial activity.
- Non-thermal plasma (NTP) is explored for synergistic anticancer strategies.
Purpose of the Study:
- To investigate the transmembrane process and mechanism of MEL activity in various cell systems.
- To evaluate the synergistic potential of combining MEL with NTP for cancer therapy.
Main Methods:
- Studied the interaction of MEL with lipid bilayers to understand its orientation and binding.
- Investigated the influence of sialic acid overexpression on MEL membrane interaction.
- Assessed the combined effects of MEL and NTP on cancer cells.
Main Results:
- Electrostatic attraction stabilizes MEL orientation on cell membranes.
- Sialic acid levels impact MEL binding and membrane stability.
- NTP reduces the required MEL dosage and its non-specific cytotoxic effects.
Conclusions:
- The study provides theoretical support for enhancing MEL's clinical applicability.
- Combining MEL with NTP offers a promising strategy for cancer treatment in plasma biomedicine.
