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Circulating Endocannabinoids in Canine Cutaneous Mast Cell Tumor.

Valentina Rinaldi¹, Fabiana Piscitelli², Andrea Boari¹

¹Department of Veterinary Medicine, Veterinary Teaching Hospital, University of Teramo, 64100 Teramo, Italy.

Animals : an Open Access Journal From MDPI

|October 26, 2024

Summary

Plasma levels of endocannabinoids (eCBs) were altered in dogs with cutaneous mast cell tumors (cMCT). Specifically, 2-arachidonoylglycerol (2-AG) was higher, while N-arachidonoylethanolamine (AEA) and N-palmitoylethanolamine (PEA) were lower in affected dogs.

Keywords:

MCT dog endocannabinoid tumor

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Published on: July 4, 2018

Area of Science:

Veterinary Oncology
Biochemistry
Canine Health

Background:

Cutaneous mast cell tumors (cMCT) are common in dogs.
The endocannabinoid system (ECS), involving endocannabinoids (eCBs), plays a role in various physiological processes.
Alterations in eCBs may be linked to tumor development.

Purpose of the Study:

To investigate plasma levels of key endocannabinoids (AEA, 2-AG, PEA, OEA) in dogs with cMCT.
To determine if eCBs can serve as biomarkers for distinguishing cMCT in dogs.
To explore the relationship between eCB levels and cMCT presence.

Main Methods:

Plasma samples were collected from 17 dogs with cMCT and 11 healthy control dogs.
Levels of four endocannabinoids (AEA, 2-AG, PEA, OEA) were quantified using established analytical techniques.
Receiver Operating Characteristic (ROC) curve analysis was used to assess the diagnostic accuracy of eCBs.

Main Results:

Dogs with cMCT exhibited significantly higher plasma 2-AG levels (p=0.0001) compared to controls.
Lower plasma levels of AEA (p=0.0012) and PEA (p=0.0075) were observed in dogs with cMCT.
2-AG demonstrated high diagnostic accuracy (0.98 AUC) for distinguishing cMCT, with 94% sensitivity and 90% specificity at a threshold of 52.75 pmol/mL.

Conclusions:

This study is the first to report altered plasma eCB levels in dogs with cMCT.
Plasmatic 2-AG, AEA, and PEA show potential as non-invasive biomarkers for cMCT diagnosis in dogs.
Further research is warranted to elucidate the role of eCBs in canine tumorigenesis.