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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
Published on: April 14, 2015
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Effectiveness of Lyoprotectants in Protein Stabilization During Lyophilization
Vinoothini Karunnanithy1, Nur Hazirah Binti Abdul Rahman1, Nur Atiqah Haizum Abdullah1
1Department of Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia.
Pharmaceutics
|October 26, 2024
Summary
Disaccharides are the most effective lyoprotectants for stabilizing proteins during freeze-drying (lyophilization). Other lyoprotectant classes show benefits when used in combination, protecting protein structure and function.
Area of Science:
- Biochemistry
- Pharmaceutical Sciences
- Materials Science
Background:
- Proteins are vital in healthcare but sensitive to physical and chemical changes.
- Lyophilization (freeze-drying) enhances protein shelf life but can compromise structural integrity.
- Lyoprotectants are crucial for preserving protein conformation during freeze-drying.
Purpose of the Study:
- To review and categorize lyoprotectants based on their effectiveness in protecting protein structures.
- To identify optimal lyoprotectant strategies for pharmaceutical applications.
Main Methods:
- Systematic literature review of 20 articles from Scopus, Web of Science, and PubMed.
- Analysis of lyoprotectant categories (sugars, polyols, surfactants, amino acids) and their interactions with proteins.
Main Results:
- Lyoprotectants, including sugars, polyols, surfactants, and amino acids, offer significant protein protection.
- Proteins are stabilized through mechanisms like hydrogen bonding, hydrophobic interactions, electrostatic interactions, and osmoprotection.
- Combinations of lyoprotectants can enhance protective effects.
Conclusions:
- Disaccharides emerge as the most effective single-agent lyoprotectants for protein stabilization.
- Optimal protein protection during lyophilization often requires combining different classes of lyoprotectants.
