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Related Experiment Video

Updated: Jun 12, 2026

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

Gradient Functionalization of Poly(lactic acid)-Based Materials with Polylysine for Spatially Controlled Cell

Viktor Korzhikov-Vlakh^1,2, Aleksandra Mikhailova^1,3, Ekaterina Sinitsyna¹

¹Institute of Chemistry, St. Petersburg, St. Petersburg State University, 198504 St. Petersburg, Russia.

|October 26, 2024

Summary

Researchers developed gradient surface modifications on PLA materials using polylysine (PLys) for controlled cell adhesion and migration, advancing tissue engineering applications.

Keywords:

biomedical materials poly(lactic acid)polylysine covalent grafting surface gradient modification thiol-ene click reaction

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Published on: January 20, 2018

Area of Science:

Biomaterials Science
Polymer Chemistry
Tissue Engineering

Background:

Gradient surface modification is crucial for controlling cell behavior in tissue engineering.
Poly(lactic acid) (PLA)-based materials offer biocompatibility but require surface functionalization for specific cell interactions.

Purpose of the Study:

To develop a method for covalent modification of PLA surfaces with polylysine (PLys) using a light-gradient-induced thiol-ene click reaction.
To create spatially controlled cell adhesion and migration on modified PLA surfaces.

Main Methods:

Fabrication of PLA-based films and modification with 2-aminoethyl methacrylate (AEMA) to introduce double bonds.
Synthesis of thiol-terminated polylysine (Cys-PLys) via ring-opening polymerization and deprotection.
Photoinduced thiol-ene click reaction under a light gradient to covalently graft Cys-PLys onto PLA-AEMA surfaces.
Characterization using NMR, Raman, XPS, and fluorescent staining.

Main Results:

Successful covalent modification of PLA films with AEMA was confirmed.
Gradient grafting of Cys-PLys onto PLA surfaces was achieved and visualized using fluorescent staining.
Demonstrated gradient-dependent adhesion and migration of HEK 293 cells on the modified surfaces.

Conclusions:

A novel method for creating gradient surface modifications on PLA using light-induced thiol-ene click chemistry was established.
The gradient surfaces effectively controlled cell adhesion and migration, showing potential for advanced tissue engineering scaffolds.