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Updated: Jun 9, 2025
Preparation of Naringenin Solution for In Vivo Application
Published on: August 10, 2021
Comparative Analysis of the Protective Effect of Naringenin on Cardiovascular Parameters of Normotensive and
Anelize Dada1, Rita de Cássia Vilhena da Silva1, Mariana Zanovello1
1Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (Univali), Itajai 88302-901, SC, Brazil.
Insights
Naringenin, a citrus flavonoid, shows protective effects against heart damage induced by isoproterenol in rats. It reduced blood pressure, clot formation, and cardiac damage, suggesting potential therapeutic benefits for myocardial infarction.
Area of Science:
- Cardiovascular Pharmacology
- Natural Product Chemistry
- Biochemistry
Background:
- Cardiovascular diseases, including myocardial infarction (MI), are leading causes of mortality worldwide.
- Medicinal plant bioactives offer promising avenues for novel therapeutic development.
- Naringenin (NAR), a flavonoid from citrus fruits, is investigated for its cardioprotective properties.
Purpose of the Study:
- To evaluate the effects of naringenin (NAR) in normotensive (NTR) and spontaneously hypertensive (SHR) rats with isoproterenol (ISO)-induced myocardial infarction (MI).
Main Methods:
- Administration of naringenin (100 mg/kg) to rats subjected to isoproterenol (85 mg/kg)-induced MI.
- Assessment of blood pressure, clot formation, aortic contractility, oxidative stress markers (GSH, LOOH), antioxidant enzyme activity (CAT, GST), and histological myocardial damage.
Main Results:
- Naringenin reduced blood pressure and clot formation, particularly in SHR rats.
- NAR restored KCl-induced aortic contractility in both NTR and SHR groups.
- Treatment with NAR improved oxidative stress markers and antioxidant enzyme activity, and reduced myocardial damage.
Conclusions:
- Naringenin exhibits significant cardioprotective potential against isoproterenol-induced cardiac damage in rat models.
- The findings suggest differential pathophysiological responses between normotensive and hypertensive rats.
- Further research is warranted to elucidate the precise mechanisms underlying naringenin's protective effects.
Abstract:
Background: Cardiovascular diseases rank as the top global cause of mortality, particularly acute myocardial infarction (MI). MI arises from the blockage of a coronary artery, which disrupts blood flow and results in tissue death. Among therapeutic approaches, bioactives from medicinal plants emerge as promising for the development of new medicines. Objectives: This study explored the effects of naringenin (NAR 100 mg/kg), a flavonoid found in citrus fruits, in normotensive (NTR) and spontaneously hypertensive (SHR) rats, both subjected to isoproterenol (ISO 85 mg/kg)-induced MI. Results: Post-treatment assessments indicated that NAR reduced blood pressure and minimized clot formation, particularly notable in the SHR group, which helps mitigate damage related to hypertension and ISO exposure. Additionally, NAR effectively restored KCl-induced contractility in the aortas of both NTR and SHR groups. NAR treatment reduced reduced glutathione (GSH) and lipid hydroperoxides (LOOH) values and recovered the activity of the antioxidant enzymes catalase (CAT) and glutathione-s-transferase (GST) in NTR groups. Moreover, myocardial damage assessed through histological analyses was reduced in groups treated with NAR. Conclusions: The results highlight significant pathophysiological differences between the groups, suggesting that NAR has protective potential against ISO-induced cardiac damage, warranting further investigation into its protective effects and mechanisms.
