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Full-Length ASFV B646L Gene Sequencing by Nanopore Offers a Simple and Rapid Approach for Identifying ASFV Genotypes.
Vivian O'Donnell1, Edward Spinard2,3, Lizhe Xu1
1Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Orient, NY 11944, USA.
Viruses
|October 26, 2024
Summary
African swine fever (ASF) is a severe viral disease. A new rapid nanopore sequencing method allows for quick characterization and genotyping of ASF virus strains, aiding in real-time outbreak investigations.
Area of Science:
- Veterinary Virology
- Molecular Epidemiology
- Genomic Sequencing
Background:
- African swine fever (ASF) is a highly contagious viral disease affecting pigs and wild boars.
- The African swine fever virus (ASFV) is classified into genotypes based on the B646L gene encoding the p72 capsid protein.
- Accurate and rapid genotyping is crucial for epidemiological tracking and understanding ASF outbreaks.
Purpose of the Study:
- To evaluate a rapid, cost-effective method for full-length B646L gene sequencing of ASFV.
- To enable real-time characterization and genotyping of ASF virus strains.
- To facilitate quicker identification of ASF origins during outbreaks.
Main Methods:
- An amplicon-based sequencing approach targeting the entire B646L gene.
- Multiplex nanopore sequencing utilizing Flongle flow cells.
- Real-time data analysis for rapid genotype determination.
Main Results:
- The developed method provides an easy, low-cost, and rapid way to sequence the full-length B646L gene.
- Successful real-time characterization and genotyping of ASF virus were achieved.
- The approach is suitable for immediate application in outbreak settings.
Conclusions:
- Nanopore sequencing of the full-length B646L gene offers a powerful tool for ASF genotyping.
- This method enhances the speed and efficiency of epidemiological surveillance for ASF.
- Real-time genotyping capabilities are essential for timely control and management of ASF outbreaks.
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