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The updated RPS 2.0 database now includes over 171,000 liquid-liquid phase separation (LLPS)-associated RNAs. This resource enhances understanding of RNA roles in biomolecular condensates and their regulation.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Liquid-liquid phase separation (LLPS) drives the formation of essential biomolecular condensates.
  • Both proteins and RNAs are critical components of these condensates, influencing physiological and pathological processes.
  • The initial RNA-Phase Separation (RPS) database was established in 2021 to catalog LLPS-associated RNAs.

Purpose of the Study:

  • To present an updated version of the RPS database, named RPS 2.0.
  • To incorporate newly identified LLPS-associated RNAs, particularly those regulated by post-transcriptional mechanisms.
  • To provide comprehensive annotations for these RNAs to facilitate research.

Main Methods:

  • Database update and expansion.
  • Integration of new high-throughput and predicted data for LLPS-associated RNAs.
  • Annotation of RNA sequence, structure, interactions, modifications, and disease relevance.

Main Results:

  • RPS 2.0 hosts 171,301 entries of LLPS-associated RNAs across 24 biomolecular condensates.
  • Data includes four evidence types (Reviewed, High-throughput, Predicted) and five event types (Expression, APA, AS, A-to-I, Modification).
  • Extensive annotations cover RNA features, interactions, modifications, and disease associations.

Conclusions:

  • RPS 2.0 is a significantly expanded resource for LLPS-associated RNAs.
  • The database will advance research into the biological functions and regulatory mechanisms of LLPS.
  • This resource supports a deeper understanding of RNA's role in biomolecular condensate formation and function.