Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Daily Rhythms and Postprandial Responses on the Plasma Metabolome.

International journal of molecular sciences·2026
Same author

Plasma protein signatures altered before and after tuberculosis diagnosis in a population-based cohort.

Clinical proteomics·2026
Same author

Companion animal owner "types" identified using a large-scale international assessment of the human-animal bond.

Frontiers in veterinary science·2026
Same author

Leveraging artificial intelligence in bioacoustics for animal health monitoring and early diagnosis in veterinary medicine.

Frontiers in veterinary science·2026
Same author

Ion Beam Imaging Shows Selective Thallium-201 Uptake in Cell Nuclei: Impact on Cellular Dosimetry and Radiotoxicity of Auger Electron Emitters.

Chemical & biomedical imaging·2026
Same author

Cellular level lipidomics of two-dimensional cultures of adherent gut epithelial cell lines confirms a metabolic switch.

The Analyst·2026

Related Experiment Video

Updated: Jun 9, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

Single-Cell Lipidomics: An Automated and Accessible Microfluidic Workflow Validated by Capillary Sampling.

Anastasia Kontiza1, Johanna von Gerichten1, Kyle D G Saunders1

  • 1School of Chemistry and Chemical Engineering, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford GU2 7XH, United Kingdom.

Analytical Chemistry
|October 26, 2024
PubMed
Summary

We developed a microfluidics method for measuring lipids in single living cells using liquid chromatography mass spectrometry (LC-MS). This accessible, high-throughput approach enables automated single-cell lipid profiling.

More Related Videos

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.8K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.5K

Related Experiment Videos

Last Updated: Jun 9, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.8K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.5K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology

Background:

  • Single-cell lipidomics is crucial for understanding cellular heterogeneity.
  • Existing methods for single-cell lipid analysis face challenges in throughput and accessibility.
  • Microfluidics offers potential for precise cellular manipulation and analysis.

Purpose of the Study:

  • To demonstrate a novel microfluidics-based approach for measuring lipids in single living cells.
  • To enable high-throughput, automated single-cell lipid profiling using accessible LC-MS instrumentation.
  • To validate the method against established single-cell lipidomics techniques.

Main Methods:

  • Development of a microfluidics system for sorting and dispensing single living cells.
  • Integration with liquid chromatography-mass spectrometry (LC-MS) for lipid analysis.
  • Optimization of protocols to minimize lipid contamination and maximize lipid identification.

Main Results:

  • Comparable lipid feature detection (around 200 lipids) to existing methods, but with higher throughput.
  • Successful automated sampling, data acquisition, and analysis of single-cell lipidomes.
  • Demonstrated a 75% increase in identified lipids through protocol optimization.

Conclusions:

  • The microfluidics-based approach provides an accessible and high-throughput method for single-cell lipid profiling.
  • This technique validates and advances the field of single-cell lipidomics.
  • The method facilitates deeper insights into cellular lipid heterogeneity.