Related Experiment Video
Updated: Jul 10, 2026
07:48
A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
Published on: September 22, 2011
22.2K
Antiapoptosis Engineering for Improved Protein Production from CHO Cells
Eric Baek1, Soo Min Noh1, Su Hyun Kim1
1Department of Biological Sciences, KAIST, Daejeon, Republic of Korea.
Methods in Molecular Biology (Clifton, N.J.)
|October 26, 2024
Summary
This study details methods for creating stable antiapoptotic cell lines to boost therapeutic protein production. By reducing cell death (apoptosis), researchers enhance protein yields and cell resilience to stress.
Area of Science:
- Biotechnology
- Cell Biology
- Biopharmaceutical Manufacturing
Background:
- Efficient production of therapeutic proteins relies on maximizing viable cell concentration over time.
- Cell death, specifically apoptosis, significantly limits protein yields in biomanufacturing.
- Strategies to enhance cell survival are crucial for improving bioprocess efficiency.
Purpose of the Study:
- To outline the methodology for constructing stable antiapoptotic cell lines.
- To demonstrate how manipulating apoptotic pathways can increase therapeutic protein yields.
- To provide a guide covering the entire process from clone selection to activity verification.
Main Methods:
- Establishing stable cell lines through genetic engineering.
- Overexpressing antiapoptotic genes or downregulating proapoptotic genes.
- Selecting high-expressing clones and verifying antiapoptotic activity.
Main Results:
- Enhanced resistance of cell lines to environmental stresses.
- Increased time integral of viable cell concentration.
- Improved final product yields for therapeutic proteins.
Conclusions:
- Constructing stable antiapoptotic cell lines is an effective strategy for enhancing therapeutic protein production.
- Genetic modification to control apoptosis leads to more robust cell lines.
- The discussed methods facilitate the development of efficient biomanufacturing processes.
Related Concept Videos
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:30Apoptosis
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size reduction of the tissue.
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
01:30Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...