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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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Electrochemical sensor for vascular endothelial growth factor based on self-assembling DNA aptamer structure.

Viktorija Liustrovaite¹, Vilma Ratautaite², Almira Ramanaviciene³

¹Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, Vilnius LT-03225, Lithuania; NanoTechnas, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, Vilnius LT-03225, Lithuania.

The Science of the Total Environment

|October 26, 2024

Summary

This study developed a novel electrochemical sensor using DNA aptamers and polypyrrole for sensitive vascular endothelial growth factor (VEGF) detection. This biosensor shows promise for early cancer diagnosis and monitoring treatment efficacy.

Keywords:

Biosensors DNA aptamers Electrochemical sensor Polypyrrole (Ppy)Screen-printed carbon electrode (SPCE)Vascular endothelial growth factor (VEGF)

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Vascular endothelial growth factor (VEGF) is crucial for early cancer detection and monitoring treatment.
Developing sensitive and selective detection methods for VEGF is essential.

Purpose of the Study:

To design and characterize an electrochemical sensor for sensitive and selective VEGF detection.
To utilize a self-assembling DNA aptamer structure integrated with polypyrrole for enhanced sensing capabilities.

Main Methods:

Electrochemical deposition of polypyrrole (Ppy) layers onto screen-printed carbon electrodes (SPCEs).
Integration of a self-assembled DNA aptamer structure within the Ppy matrix ((DNA aptamer)/Ppy).
Detection of VEGF using pulsed amperometric detection (PAD) and analysis via Langmuir isotherm model.

Main Results:

The developed (DNA aptamer)/Ppy sensor demonstrated enhanced performance compared to bare Ppy.
Achieved a high sensitivity with a limit of detection (LOD) of 0.21 nM for VEGF.
The sensor showed promising results for in vitro applications.

Conclusions:

The novel electrochemical biosensor is effective for sensitive and selective VEGF detection.
The sensor holds significant potential for early cancer diagnostics and monitoring cancer treatment.
The developed method enables rapid screening of patient samples.